|
page last updated -
|
 |
 |
 |
 |
 |
| The DBC | FACULTY | RESEARCH THEMES | FACILITIES | INFO |
|
|
|
|
 |
|
|
Fluorescence correlation spectroscopy (FCS) is a powerful tool for studying the dynamic properties of diffusion and chemical reaction rates. Significant advances in this technology were acheived by integration of confocal and two-photon techniques. These greatly improved the signal-to-noise ratio of FCS and reduced the measured volume element to less than one femtoliter. These developments in FCS technology make it possible to observe the dynamics and concentrations of macromolecules directly in living cells. |
|
| To find additional information about methods for FCS, confocal microscopy using LSM510META, multi-photon lasers, objectives, how to separate different spectra of fluorophores, how to get around problems with "bleed-through" of signal and much more, visit the OBC's confocal microscopy resource page, or contact Dr. Michelle Digman, the Director of the Optical Biology Core Facility. | |