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| The DBC | FACULTY | RESEARCH THEMES | FACILITIES | INFO |
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The BD FACSCalibur™ Flow Cytometry System is a four-color, dual-laser, benchtop system designed to support a wide range of both basic and clinical research. The instrument can detect three fluorochromes excited by the 488nm laser and one fluorochrome excited by the 635nm laser. Fluorochromes that can be analyzed on this instrument include: - 488nm excitable: FITC, PE, PerCP, PE-Cy5, PE-Cy5.5, PE-Cy7, PerCP-Cy5.5, CFSE, GFP, Alexa 488, PI, 7-AAD, DRAQ 5, Acridine Orange, PKH-26, Fura Red, Fluo-3, DiI, DCFDA, Sytox Green, Cy3, Ethidium Bromide, DHR123, TMR, JC-1 - 635nm excitable: APC, DRAQ 5, TO-PRO3, Cy5, Alexa 633, Alexa 647 The emission filters are static, and are as follows: - Emissions from the 488nm excitation FL1 - 530/30 nm (515-545 nm) FL2 - 585/42 nm (564-606 nm) FL3 - 670 nm LP (>670 nm) - Emissions from the 635 nm excitation FL4 - 661/16 nm (653-669 nm) Consult the facility for fluorochrome combination feasibility and necessary controls prior to designing your experiment. For more information, including rates for service and to schedule cell cytometry or sorting, please contact Ida Theodor, the Manager of the Flow Cytometry Facility in the Center for Immunology. |
